<i>MEFV</i> Gene Mutation Analysis in Children with Immunoglobulin A Vasculitis and Its Effects on Clinical Manifestations: A Big Series from a Tertiary Center
PDF
Cite
Share
Request
Original Article
P: 82-91
March 2024

MEFV Gene Mutation Analysis in Children with Immunoglobulin A Vasculitis and Its Effects on Clinical Manifestations: A Big Series from a Tertiary Center

Med Bull Haseki 2024;62(2):82-91
1. Goztepe Prof. Dr. Suleyman Yalcin City Hospital, Clinic of Pediatrics, Istanbul, Turkey
2. Altinova State Hospital, Clinic of Pediatrics, Yalova, Turkey
3. Golbasi District Health Directorate, Ankara, Turkey
4. Duzce University Faculty of Medicine, Department of Pediatric Rheumatology, Duzce, Turkey
No information available.
No information available
Received Date: 26.10.2023
Accepted Date: 03.03.2024
Publish Date: 08.05.2024
PDF
Cite
Share
Request

ABSTRACT

Aim:

Immunoglobulin A vasculitis (IgAV) is the most common vasculitis of childhood, but its pathogenesis is largely unknown, despite evidence pointing to various environmental and genetic factors. We investigated the frequency of MEFV gene mutations that are considered in the pathogenesis and their effect on the clinical features of patients with IgAV.

Methods:

The study included 244 children diagnosed with IgAV, who underwent MEFV gene analyses. We recorded the demographic and clinical characteristics of the patients, along with their laboratory results. We grouped the patients based on the presence of MEFV gene mutations and MEFV variants.

Results:

At least one MEFV mutation was detected in 89 (36.5%) patients, with E148Q being the most common (n=31, 34.8%). Age at diagnosis and the frequency of hematuria and recurrence were significantly greater among patients with MEFV mutations (p=0.043, p=0.008, and p=0.009, respectively). Serum IgA levels were significantly higher in patients with the M694V mutation (p=0.040).

Conclusion:

The presence of MEFV mutations, particularly E148Q and M694V, could be associated with the development and clinical course of IgA vasculitis.

Keywords:
Children, hematuria, IgA vasculitis, MEFV gene, recurrence

Introduction

Immunoglobulin A vasculitis (IgAV), characterized by IgA and immune complex deposition in small vessels, is the most common vasculitis worldwide (1). The primary clinical manifestations of the disease include non-thrombocytopenic palpable purpura, joint gastrointestinal (GI) tract involvement, and renal involvement (2). The etiopathogenesis of IgAV remains unclear, and genetic and environmental factors are thought to contribute to the disease (3). Nonetheless, various recent publications have focused on genetic factors, exemplified by the demonstration of the roles of polymorphisms in genes encoding cytokines and cell adhesion molecules (4-6).

Familial Mediterranean Fever (FMF) is an autoinflammatory disorder characterized by recurrent attacks of fever and serositis. It is caused by mutations in the Mediterranean Fever (MEFV) gene that encode pyrin, a protein involved in apoptosis, inflammation, and the secretion of cytokines (7,8). Researchers have identified over 350 mutations in the MEFV gene. M694V, M694I, M680I, V726A, and E148Q are the most frequently detected mutations in patients with FMF in Turkey (9,10).

Researchers mentioned that FMF may co-exist with various inflammatory diseases, including IgAV, juvenile idiopathic arthritis, Behcet’s disease, inflammatory bowel disease, and polyarteritis nodosa. It has been established in the literature that MEFV gene mutations lead to an uncontrollable inflammatory reaction (11-13). Immunoglobulin A vasculitis was reported as the most common coexisting vasculitis with FMF. It has been speculated that IgAV patients with MEFV gene mutations have greater clinical severity and worse laboratory findings due to an exaggerated inflammatory response (11,14,15). However, the available literature on this topic is limited because of the investigation of only a few common mutations.

In the present study, we aimed to investigate the frequency of MEFV gene mutations in children with IgAV and to assess potential relationships between these mutations and the clinical course and laboratory findings.

Methods

Compliance with Ethical Standards

Ethical approval was obtained from the Istanbul Medeniyet University, Goztepe Training and Research Hospital Clinical Research Ethics Committee (approval no.: 2023/0059, date: 25.01.2023) before the experiment was started, and the experiment was conducted in accordance with the principles set forth in the Helsinki Declaration. Written informed consent was obtained from the legal guardians of the children.

Study Design and Population

The present study was a retrospective, cross-sectional study that was performed in a single tertiary healthcare institution from January 2005 to December 2021. Five hundred eighteen children aged 18 years who were admitted (and followed for at least 6 months) to the pediatric and pediatric rheumatology departments with the diagnosis of IgAV were assessed for inclusion in the study. Immunoglobulin A vasculitis diagnosis was made according to the consensus criteria put forth by the European League Against Rheumatism and the Pediatric Rheumatology European Society (EULAR/PRINTO/PRES 2010) (16).

Among these 518 children, we excluded subjects diagnosed with FMF before the onset of IgAV as well as those with additional chronic diseases (comorbidities), insufficient follow-up duration (<6 months), unperformed MEFV gene analyses, and incomplete data. A total of 244 children diagnosed with IgAV were included in the analyses. Criteria for inclusion and exclusion in the study are shown in Figure 1.

Figure 1

Data Collection and Disease Definitions

The patients’ demographic, clinical, and laboratory data were recorded from their medical records. In all patients, symptoms, signs, and organ involvement, such as the skin, GI tract, joints, kidneys, testicles, and central nervous system, were recorded on admission and during follow-up. Cutaneous findings consisted of characteristic palpable purpura and subcutaneous edema. Gastrointestinal manifestations included vomiting, abdominal pain, invagination, and GI bleeding-defined as melena, hematochezia, or occult blood in the stool. Involvement of more than one joint but fewer than five joints was considered to demonstrate the presence of oligoarthritis. All patients were followed up for a minimum period of 6 months for renal involvement. Hematuria was defined as the presence of >5 red blood cells per high-power field in a urine examination. Mild proteinuria was defined as a spot urine protein-to-creatinine ratio of 0.2 mg/mg, whereas nephrotic proteinuria was defined in patients with a ratio of >3-3.5 mg/mg or those with absolute levels of >40 mg/m2/h. Renal biopsy was performed in selected cases when patients presented with nephrotic-range proteinuria, persistent proteinuria of 4-40 mg/m2/h for more than 3 months, and/or renal impairment. Skin biopsies were performed on selected IgAV patients with atypical rashes.

Patient Management

Treatment modalities, including hydration, non-steroidal anti-inflammatory drugs (NSAIDs), and the need for corticosteroid therapy and colchicine, were recorded. Treatment was planned according to existing symptoms; we routinely prescribed bed rest and NSAIDs for arthralgia and mild abdominal discomfort. Systemic steroid treatment was reserved for severe GI involvement, including severe abdominal pain, GI bleeding (GIS), progressive renal disease, and scrotal edema.

Biochemistry and Genetic Analyses

White blood cell (WBC) and platelet (PLT) count (109/L), hemoglobin (Hb) level (g/dL), C-reactive protein (CRP) (mg/dL), Westergren erythrocyte sedimentation rate (ESR) (mm/hr), stool blood analysis, and other laboratory parameters such as anti-streptolysin O (ASO), C3 and C4 complement levels, and serum IGA levels were determined by standard laboratory methods at the time of diagnosis. Leukocytosis was defined if the WBC count was ≥10,000/mm3. Thrombocytopenia was accepted as a PLT level of <150,000. Elevated levels of CRP, ESR, ASO, C3, and C4 were defined as >5 mg/dL, >15 mm/h, >200 IU/mL, >170 mg/dL, and >44 mg/dL, respectively. Blood cultures, hepatitis B, hepatitis C, or human immunodeficiency virus infection serology were only performed when deemed necessary by the attending physician. Disease-related complications included the development of hypertension, invagination, and convulsions. The recurrence of disease was recorded in the medical files of the patients. Recurrence was defined as disease activation after a period of at least 3 months without signs or symptoms.

All patients in the cohort were analyzed for sequence variants in exons 2, 3, 5, and 10 of the MEFV gene. For the main comparative analyses, the study population was divided into two main groups: “MEFV mutation carriers” (patients with mutations in at least one allele; heterozygous, homozygous, and compound heterozygous) and "non-carriers". Carrier patients were divided into three further subgroups according to their carrier status: p. M694V carriers, E148Q carriers, and “other mutation” carriers (Figure 1). We compared the demographic, clinical, and laboratory findings between the groups.

Statistical Analysis

All analyses were performed using the SPSS 23.0 statistical software package (IBM SPSS Statistics). Categorical variables were expressed as numbers (n) and percentages, whereas continuous variables were summarized as mean with standard deviation or as median with minimum and maximum where appropriate. Chi-square tests were used to compare categorical variables between groups. The normality of the distribution for continuous variables was tested using the Kolmogorov-Smirnov test (Lilliefors correction). For continuous variables that had a normal distribution, two-group comparisons were performed using the Independent Samples t-test (variances were assessed according to Levene’s test, and p-values were determined according to those results). For >2-group comparisons, we used ANOVA. In the absence of a normal distribution, we used the Mann-Whitney U test for two-group comparisons and the Kruskal-Wallis test for >2-group comparisons. For the pairwise corrections of the ANOVA and Kruskal-Wallis tests, we used the Bonferroni correction, given that statistical assumptions were fulfilled. The statistical level of significance for all tests was defined as a p-value of <0.05. For power analysis, the G-Power 3.1.9.7 program was used. The power of the study was found to be 0.98.

Results

A total of 244 of the 518 children with IgAV were included in the analyses. Table 1 summarizes the demographic, clinical, and laboratory characteristics of patients with IgAV.

Table 1

Distribution of MEFV Mutations in Patients with IgAV

At least one MEFV mutation was detected in 89 (36.5%) of the 244 patients with IgAV included in the study. Most were heterozygous mutations (n=71, 79.8%). E148Q was the most common mutation (n=31, 34.8%) among all MEFV mutations. One patient had both E148Q and M694V mutations and was not included in the comparison analyses to avoid errors in interpretations. The genotype distribution of the patients is depicted in Table 2.

Table 2

Comparison of Clinical Characteristics and Laboratory Findings Between MEFV Mutation Carriers and Non-Carrier Patients

Clinical and laboratory parameters were compared between MEFV mutation carriers and non-carrier patients with IgAV. Age at diagnosis, frequency of hematuria, and disease recurrence were significantly greater in MEFV mutation carrier patients than in non-carriers (p=0.043, p=0.008, p=0.009) respectively. Although patients with MEFV mutations had relatively higher WBC, CRP, and ESR levels, there was no statistically significant difference between the groups (Table 3).

Table 3

Comparison of Demographic, Clinical, and Laboratory Findings of Patients with IgAV According to the Presence of MEFV Gene Variants

The relationship between the different MEFV gene variants (E148Q, M694V, and “others”) and clinical characteristics is shown in Table 4. In terms of clinical characteristics, vomiting was more frequent in patients with mutations other than E148Q and M694V (p=0.026). None of the patients with E148Q and M694V mutations experienced vomiting. In terms of laboratory findings, only the serum IgA level was significantly higher in patients with the M694V mutation (p=0.040).

Table 4

Discussion

Association of IgAV with MEFV Mutations and Their Variants

The association of vasculitis with FMF has been extensively reported in previous studies (11-13,17). The MEFV gene encodes the pyrine protein, which plays an essential role in inflammatory pathways by decreasing inflammation; hence, the mutated protein causes uncontrolled inflammation and predisposes to the occurrence of vasculitis (including IgAV) (12,18). Previous research indicates that if IgAV is diagnosed in a patient belonging to an ethnic/racial group in which FMF is frequent, physicians should assess the presence of FMF symptoms (19). It has been established that the prevalence of MEFV gene mutations in children with IgAV is higher than that in the general population, ranging from 21% to as high as 50.7%, and it has been shown that MEFV mutations can affect the clinical and laboratory findings of IgAV (14,20-26). In a previous study, Ozçakar et al. (20) reported that 27 out of 80 patients (34%) with IgAV had MEFV gene mutations. Additionally, in a recent study from Turkey, Acarı et al. (15) reported that MEFV gene mutations were found in 25 out of 47 (53%) patients with IgAV, and this rate was significantly higher than that in the general Turkish population (27,28). In another study from Israel, Gershoni-Baruch et al. (21) reported that 27% of 52 patients with IgAV had at least one MEFV mutation. In contrast, in a recent literature review, Yokoyama et al. (29) mentioned that only five patients with IgAV-carrier MEFV mutations appear in Japan. In the current study, we also found that the prevalence of MEFV mutations in IgAV patients was significantly higher (n=89, 36.5%) than in the normal population and mostly demonstrated a heterozygous nature (n=71, 79.8%). The high prevalence of mutations in our study might be due to ethnic or racial predisposition, but it could also be explained by the likelihood of parental consanguinity. However, data concerning consanguinity were not assessed.

Research assessing the associations between MEFV genetic variants and IgAV has described various findings (20,21). Ozçakar et al. (20) showed that the frequencies of M694V and E148Q mutations were 20% (16 out of 80 patients) and 3.8% (3 out of 80 patients), respectively, while they were reported as 3% and 12%, respectively, in the healthy Turkish population (28). Additionally, Acarı et al. (15) reported that M694V (25%, 12 out of 47 patients), R202Q (17%, 8 out of 47 patients), and E148Q (11%, 5 out of 47 patients) were the most common detectable variants in children with IgA (15). Thus, the authors concluded that the M694V mutation was a more important predisposing factor for IgAV development, which has been supported by other studies (20,22,30). However, in another study from Turkey, it was reported that while the frequency of the M694V mutation carrier in 76 patients with IgAV was 11.7%, the frequency of the E148Q mutation carrier was 9.1% in those patients (31). Another study found that 34.8% of Turkish children with IgAV had the E148Q mutation, which is a higher frequency than that of the general Turkish population (32). In a study from China, none of the IgAV patients were found to carry the M694V mutation (33). In our study, the E148Q mutation was the most common allele; however, the frequency of the M694V mutation was also similar, indicating consistency with most of the literature.

Clinical Significance of MEFV Mutations and Variants in Patients with IgAV

It was reported earlier that MEFV mutations affect the clinical and laboratory presentation of IgAV in populations in which FMF is common (20,22,25,33,34). However, studies comparing carriers and non-carriers of MEFV mutations have reported conflicting results. In some of those studies, it was found that there was no relationship between the presence of MEFV carriers and disease characteristics in analyses including laboratory parameters, complications, outcomes, and treatment-related needs of patients (21,23,31,34,35). On the other hand, Ozçakar et al. (20) found that IgAV patients with MEFV carriers were younger and that MEFV mutations could affect clinical symptoms. Cakici et al. (14) demonstrated that arthritis, bowel angina, scrotal involvement, and recurrence were more common in patients with MEFV mutation positivity. In a study from Egypt, higher frequencies of arthritis, abdominal pain, GIS, hypertension, anemia, proteinuria, fecal occult blood, and recurrence were found in patients with MEFV mutations (36). Bayram et al. (22) reported that the frequency of scrotal involvement and WBC, ESR, CRP, and serum Ig levels were significantly higher in patients with MEFV mutations. Several other studies have shown significantly elevated acute phase reactants among mutation carriers (20,31,34).
Altug et al. (34) showed that ESR and CRP levels were significantly higher in patients with the IgAV-carrier MEFV mutation. Additionally, they noted that GI and joint involvement and subcutaneous edema were more common in IgAV patients carrying the MEFV mutation. Prior research has revealed that physicians should be aware of the possibility of FMF in children with intussusceptions, lower hemoglobin, higher serum IgA, and elevated PLT count; however, the study could not identify any effects of the MEFV mutations on recurrence rate (25). Interestingly, Gershoni-Baruch et al. (21) found that the recurrence rate was twofold higher in patients with two mutated alleles on MEFV compared with those without mutations, although statistical analyses were non-significant. On the other hand, in a recent study, Acarı et al. (15) found that disease relapse was significantly higher in IgA patients who were MEFV carriers than non-carriers. They also mentioned that Hb levels were lower and PLT count and CRP levels were higher in IgAV patients who had MEFV carriers (15). In comparison to prior studies, we found that mutation carriers were significantly older than non-carriers, and our results revealed that the presence of MEFV mutations was significantly associated with a higher frequency of hematuria and recurrence. White blood cell, CRP, and ESR levels were similar in carriers and non-carriers, similar to a previous report by Dogan et al. (31). Further studies are needed to determine the effects of MEFV mutations on the laboratory characteristics of patients with IgAV.

Regarding the variants (subgroup analysis) and their clinical effects, the M694V variant was found to be associated with the clinical and laboratory findings of patients with IgAV in a previous study from Turkey (22). Ozçakar et al. (20) demonstrated that the presence of edema, arthritis, and urogenital involvement was more common in patients with M694V mutations, although E148Q mutations had no clinical significance in patients with IgAV. In later years, Bayram et al. (22) found similar results to those of Ozçakar et al. (20) in their study. A study involving Chinese patients found that the E148Q variant was associated with the severity of disease, specifically with joint abnormalities; however, the researchers did not observe any significant effects of the E148Q variant on the analyzed laboratory parameters (IgA, CRP, C3, and C4) (33). In another study from Turkey, Cakici et al. (14) reported that although MEFV mutations were influential on the clinical characteristics of IgAV, variants of MEFV were not found to have any effect on the clinical course of IgAV. Acarı et al. (15) reported that scalp edema, elevated CRP levels, and disease recurrence were more common in patients with IgAV who were carriers of the M694V mutation. On the other hand, they mentioned that there was no significant relationship between the long-term prognosis of the disease and renal involvement or the presence of MEFV mutations (15). In our study, we could not find any significant results regarding the effect of the E148Q mutation in any clinical findings, but serum IgA levels were found to be significantly higher in patients with the E148Q variation, which is in contrast to the findings of the study from China. In addition, we found that vomiting was only present among patients with “other” mutations. However, this result needs further support in larger cohorts because of the limited patient counts in this study. The E148Q variant has been considered a genetic marker in some studies (33); however, to draw conclusions regarding this matter, the functional role(s) of the E148Q variant in IgAV should be elucidated.

Study Limitations

There are some limitations to our study. First, the lack of a prospective design is a major limitation of our study. Second, we only investigated 12 well-known MEFV variants instead of identifying all the variants. Finally, the clinical spectrum of IgAV is similar between isolated IgAV and FMF-associated IgAV; therefore, particularly in some cases, IgAV may be an initial symptom of FMF. Despite these limitations, the strength of our study is that it includes one of the largest numbers of patients among published studies to date. The potential for such a complex relationship between these conditions demonstrates the need for prospective studies including IgAV patients (with or without MEFV mutations) in which longitudinal analyses are performed in larger populations.

Conclusion

Our results showed that MEFV mutations (especially E148Q and M694V mutations) are more frequent in IgAV patients compared with the general population, and the presence of those mutations seems to have some effects on the clinical features of IgAV patients, as demonstrated by results concerning hematuria and recurrence. Therefore, patients with IgAV, especially older children, should be followed more carefully regarding FMF development. In addition, closer follow-up for hematuria and recurrence appears to be necessary for patients with IgAV who carry those mutations. However, the different results and clinical effects observed in other studies indicate the need for further research.

Ethics

Ethics Committee Approval: Ethical approval was obtained from the Istanbul Medeniyet University, Goztepe Training and Research Hospital Clinical Research Ethics Committee (approval no.: 2023/0059, date: 25.01.2023).

Informed Consent: Written informed consent was obtained from the legal guardians of the children.

Authorship Contributions

Surgical and Medical Practices: S.Y., Z.K., M.E., Concept: S.Y., M.E., Design: S.Y., M.E., Data Collection or Processing: S.Y., Z.K., Analysis or Interpretation: O.O., Literature Search: S.Y., Z.K., Writing: S.Y., Z.K., M.E.

Conflict of Interest: No conflicts of interest were declared by the authors.

Financial Disclosure: This study received no financial support.

References

1
Gardner-Medwin JM, Dolezalova P, Cummins C, Southwood TR. Incidence of Henoch-Schönlein purpura, Kawasaki disease, and rare vasculitides in children of different ethnic origins. Lancet 2002;360:1197-202.
2
Saulsbury FT. Henoch-Schönlein purpura in children. Report of 100 patients and review of the literature. Medicine (Baltimore) 1999;78:395-409.
3
He X, Yu C, Zhao P, et al. The genetics of Henoch-Schönlein purpura: a systematic review and meta-analysis. Rheumatol Int 2013;33:1387-95.
4
Brogan PA. What’s new in the aetiopathogenesis of vasculitis? Pediatr Nephrol 2007;22:1083-94.
5
López-Mejías R, Castañeda S, Genre F, et al. Genetics of immunoglobulin-A vasculitis (Henoch-Schönlein purpura): An updated review. Autoimmun Rev 2018;17:301-15.
6
Song Y, Huang X, Yu G, et al. Pathogenesis of IgA vasculitis: an Up-To-Date review. Front Immunol 2021;12:771619.
7
Lidar M, Livneh A. Familial Mediterranean fever: clinical, molecular and management advancements. Neth J Med 2007;65:318-24.
8
French FMF Consortium. A candidate gene for familial Mediterranean fever. Nat Genet 1997;17:25-31.
9
Yildiz M, Adrovic A, Tasdemir E, et al. Evaluation of co-existing diseases in children with familial Mediterranean fever. Rheumatol Int 2020;40:57-64.
10
Tunca M, Akar S, Onen F, et al. Familial Mediterranean fever (FMF) in Turkey: results of a nationwide multicenter study. Medicine (Baltimore) 2005;84:1-11.
11
Ayaz NA, Tanatar A, Karadağ ŞG, Çakan M, Keskindemirci G, Sönmez HE. Comorbidities and phenotype-genotype correlation in children with familial Mediterranean fever. Rheumatol Int 2021;41:113-20.
12
Ozdel S, Coşkuner T, Demirkan F, et al. Inflammatory comorbidities ın the largest pediatric Familial Mediterranean fever cohort: a multicenter retrospective study of Pediatric Rheumatology Academy (PeRA)-Research Group (RG). Clin Rheumatol 2024;43:407-13.
13
Abbara S, Grateau G, Ducharme-Bénard S, Saadoun D, Georgin-Lavialle S. Association of Vasculitis and Familial Mediterranean Fever. Front Immunol 2019;10:763.
14
Cakici EK, Kurt Şükür ED, Özlü SG, et al. MEFV gene mutations in children with Henoch-Schönlein purpura and their correlations-do mutations matter? Clin Rheumatol 2019;38:1947-52.
15
Acarı C, Bayram MT, Yıldız G, Kavukçu S, Soylu A. Effect of MEFV variants on the presentation and clinical course of Henoch-Schonlein Purpura in Children? J DEU Med 2023;36:245.
16
Ozen S, Ruperto N, Dillon MJ, et al. EULAR/PReS endorsed consensus criteria for the classification of childhood vasculitides. Ann Rheum Dis 2006;65:936-41.
17
Atas N, Armagan B, Bodakci E, et al. Familial Mediterranean fever is associated with a wide spectrum of infammatory disorders: results from a large cohort study. Rheumatol Int 2020;40:41-8.
18
Flatau E, Kohn D, Schiller D, Lurie M, Levy E. Schönlein-Henoch syndrome in patients with familial Mediterranean fever. Arthritis Rheum 1982;25:42-7.
19
Cattan D. MEFV mutation carriers and diseases other than familial mediterranean fever: proved and non-proved associations; putative biological advantage. Current Drug Targets Inflamm Allergy 2005;4:105-11.
20
Ozçakar ZB, Yalçinkaya F, Cakar N, et al. MEFV mutation modify the clinical presentation of Henoch-Schönlein purpura. J Rheumatol 2008;35:2427-9.
21
Gershoni-Baruch R, Broza Y, Brik R. Prevalence and significance of mutations in the familial Mediterranean fever gene in Henoch-Schönlein purpura. J Pediatr 2003;143:658-61.
22
Bayram C, Demircin G, Erdoğan O, Bülbül M, Caltik A, Akyüz SG. Prevalence of MEFV gene mutations and their clinical correlations in Turkish children with Henoch-Schönlein purpura. Acta Paediatr 2011;100:745-9.
23
Can E, Kılınç Yaprak Z, Hamilçıkan Ş, Erol M, Bostan Gayret Y Özgül Yiğit Ö. MEFV gene mutations and clinical course in pediatric patients with Henoch-Schönlein purpura. Arch Argent Pediatr 2018;116:385-91.
24
Yilmaz E, Ozen S, Balci B, et al. Mutation frequency of Familial Mediterranean Fever and evidence for a high carrier rate in the Turkish population. Eur J Hum Genet 2001;9:553-5.
25
Ekinci RMK, Balci S, Bisgin A, et al. MEFV gene variants in children with Henoch-Schönlein purpura and association with clinical manifestations: a single-center Mediterranean experience. Postgrad Med 2019;131:68-72.
26
Bonyadi M, Younesi M, Rafeey M, Sadeghi Shabestari M, Mortazavi F. MEFV mutations in Iranian Azari Turkish patients with Henoch-Schönlein purpura. Turk J Med Sci 2016;46:967-71.
27
Soylemezoglu O, Kandur Y, Gonen S, et al. Familial Mediterranean fever gene mutation frequencies in a sample Turkish population. Clin Exp Rheumatol 2016;34:97-100.
28
Yılmaz E, Özen S, Balcı B, et al. Mutation frequency of familial Mediterranean fever and evidence for a high carrier rate in the Turkish population. Eur J Hum Genet 2001;9:553-5.
29
Yokoyama T, Sakumura N, Inoue N, Matsuda Y, Wada T. IgA vasculitis in Japanese patients harboring MEFV mutations: a case report and review of the literature. Cureus 2023;15:e34876.
30
Nikibakhsh AA, Houshmand M, Bagheri M, Zadeh HM, Rad IA. MEFV gene mutations (M694V, V726A, M680I, and A744S) in Iranian children with Henoch-Schönlein purpura. Pneumologia 2012;61:84-7.
31
Dogan CS, Akman S, Koyun M, Bilgen T, Comak E, Gokceoglu AU. Prevalence and significance of the MEFV gene mutations in childhood Henoch-Schönlein purpura without FMF symptoms. Rheumatol Int 2013;33:377-38.
32
Durmus D, Alayli G, Cengiz K, Yigit S, Canturk F, Bagci H. Clinical significance of MEFV mutations in ankylosing spondylitis. Joint Bone Spine 2009;76:260-4.
33
He X, Lu H, Kang S, et al. MEFV E148Q polymorphism is associated with Henoch-Schönlein purpura in Chinese children. Pediatr Nephrol 2010;25:2077-82.
34
Altug U, Ensari C, Sayin DB, Ensari A. MEFV gene mutations in Henoch Schonlein purpura. Int J Rheum Dis 2013;16:347-51.
35
Salah S, Rizk S, Lotfy HM, El Houchi S, Marzouk H, Farag Y. MEFV gene mutations in Egyptian children with Henoch-Schonlein purpura. Pediatr Rheumatol Online J 2014;12:41.
36
Salah S, Rizk S, Kaddah A, Houchi S, Khalifa I, Zaid W. Subclinical Familial Mediterranean Fever and MEFV gene polymorphisms in Henoch-Schnlein purpura children: Relation to the clinical and laboratory characteristics of the disease. Egypt Rheumatol 2016;38:327-32.