Original Article

To Analyse Effect of Tracheal Occlusion in the Development of Lung in Nitrofen Induced Congential Diaphragmatic Hernia Model


  • Emrah Aydın
  • Emre Yener
  • Nil Üstündağ

Received Date: 07.12.2015 Accepted Date: 10.01.2016 Med Bull Haseki 2016;54(2):62-66


Congenital daphragmatic hernia is a congenital abnormality with high morbidity and mortality. In our study, we aimed to analyse the effect of tracheal occlusion in lung development in nitrofen-induced congenital daphragmatic hernia.


Wistar albino rats of the same age were mated. The rats, in whom the presence of sperm in vaginal smear was observed, were included in the study. It was zero day of pregnancy. At the ninth day of pregnancy, 100 mg nitrofen was introduced to pregnant rats by gavage and at the 18th day of pregnancy, laparotomy and hysterotomy were performed. Tracheal occlusion was performed in one of two fetuses. At the 22nd day of pregnancy, the lungs of the fetuses were excised. Lung development was analysed histopathologically.


Ten (59%) of 17 fetuses had congenital daphragmatic hernia. Five of them were in control group. The defect was right-sided in five of the subjects, left-sided in three, and bilateral in two. Lung development was observed to be stopped in pseudoglandular subjects in two of the fetuses, in pseudoglandular-early canalicular stage in two, and early canalicular in one of the fetuses in control group while late canalicular-early saccular in two, saccular in one and early canalicular in two of the subjects in surgical group.


Nitrofen-induced congenital daphragmatic hernia is an efficient model, but it cannot simulate Bochdalek’s hernia. Tracheal occlusion increases lung development.

Keywords: Congenital diaphragmatic hernia, 2,4-dichlorophenyl-p-nitrophenyl ether, tracheal occlusion

Full Text (Turkish)